Separation of Molar Weight-Distributed Polyethylene Glycols by Reversed-Phase Chromatography—II. Preparative Isolation of Pure Single Homologs

The isolation of single homologs polyethylene glycol by preparative reversed-phase chromatography is investigated. A thermodynamic model developed accurately previously describes the retention times individual as function their size, temperature, and mobile phase composition under linear, diluted conditions. extended to predict limiting for linear gradient operation in applications. Isocratic gradient-based separations are studied strongly overloaded Baseline separation up 3000 g/mol demonstrated. Quantitative production pure molar weights 1000 was performed using an automated setup.